Extraction and Estimation of Chlorophyll from Medicinal …
characterized by UV-Visible spectroscopy. Concentration of chlorophyll a and b was calculated using Arnon method. Chlorophyll content was higher in Mimosa pudica than other medicinal plants which are used in this study. Keywords: Chlorophyll a, chlorophyll b, Medicinal plants, UV- Visible spectroscopy, Mimosa pudica. 1. Introduction
The Minimum Inhibitory Concentration of Antibiotics ...
The MIC value is the lowest concentration of an antibiotic at which bacterial growth is completely inhibited. In the agar dilution method, growth of 1–2 colonies or faint haze are disregarded [7,8]. In the broth microdilution method, for certain antibiotics, separate rules for reading the MIC value are used, including for the following:
Micronutrients: Functions, Sources and Application …
Selected Nutrient Element Adequate Concentrations and Ranges in Plants (dry weight basis). Element Symbol Range of Concentrations Adequate Concentration Nickel Ni 0.05-5 ppm 0.05 ppm Molybdenum Mo 0.10-10 ppm 0.10 ppm Cobalt Co 0.05-10 ppm 0.10 ppm Copper Cu 2-50 ppm 6 ppm Zinc Zn 10-250 ppm 20 ppm Sodium Na 0.001-8 % 10 ppm
A universal method for high-quality RNA extraction from ...
The RNA extraction methods are, Ambion TRIZOL (a), RNeasy Plant Mini Kit (Qiagen) (b), Method of Furtado 6 (c) CTAB-LiCl method (d), and modified SDS-LiCl method (e). The absorbance peaks at 220 ...
Optimization of the Method for Chlorophyll Extraction in ...
acetone method, is the mostly recognized assay for chlorophyll determination in plants because of its simplicity, sensitivity, and safety compared with the DMSO method and the DMF method (Aminot and Rey 2001, Porra 2002). In the acetone method, chlorophylls in vascular plants are often extracted with 80% aqueous acetone, and the
A New Method for the Determination of Sucrose ...
Analytical chemistry is a set of procedures and techniques used to identify and quantify the composition of a sample of material. It is also focused on improvements in experimental design and the creation of new measurement tools. Analytical chemistry has broad applications to forensics, medicine, science, and engineering. The objective of this study is to develop a new …
TOTAL PHENOLIC CONTENT, FLAVONOID …
The ability of the plant extract to scavenge DPPH free radicals was assessed by the standard method (Tekao et al., 1994), adopted with suitable modifications (KUMARASAMY et al., 2007). The stock solution of extracts were prepared in methanol to achieve the concentration of 1 mg/ml. Dilutions were made to obtain concentrations of 500, 250, 125,
Resazurin-based 96-well plate microdilution method for …
concentration incorporated into the plate is 25 mg ml-1 and the lowest achieved through double serial dilution is 0.05 mg ml-1. Column 7 shows no colour changes therefore concentration of biosurfactant in that column was taken as the MIC value. The range of biosurfactant concentration in the wells was 25–0.05 mg ml-1
METHODS OF PREPARATION PLANT GROWTH …
PLANT GROWTH REGULATORS ARE FOR LABORATORY USE, PLANT TISSUE CULTURE MEDIA PREPARATION, AND PLANT RESEARCH PURPOSES ONLY. THEY ARE NOT FOR USE AS PLANT GROWTH REGULATORS ON DEVELOPED PLANTS. THEY ARE NOT FOR DRUG OR USE. PRODUCT USE Auxins: Auxins are generally used …
Evaluation of Methods for the Concentration and …
Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment efficiency. In raw sewage human waste is mixed with , industrial and drainage water, and virus particles are, therefore, only found in low concentrations. This necessitates a step of sample concentration to allow for …
Dry matter content as a measure of dry matter ...
concentration. Methods We present data from both plants growing in the field and plants grown under controlled conditions in hydroponic sand culture. Taxonomy follows Marie-Victorin (1964). The field data set consisted of leaves from 28 species, of which 12 were herbaceous, eight were trees, five were forest understory shrubs
Measuring Soluble Ion Concentrations (Na+, K+, Cl−) in ...
Abstract. The control of Na + and Cl − uptake from soils, and the partitioning of these ions within plants, is an essential component of salinity tolerance. Genetic variation in the ability of roots to exclude Na + and Cl − from the transpiration stream flowing to the shoot has been associated with salinity tolerance in many species. The maintenance of a high uptake of K + is …
A comprehensive comparison of four methods for …
The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis …
Effect of kinetin concentration and methods of application ...
Moreover, significant increases were recorded in the number of open bolls/plant, boll weight, lint and seed indices, seed cotton yield/plant, and seed cotton and lint yields/plot. The highest means were obtained at 5 mg kinetin l(-1) concentration and under method C of application.
Concentration of bioaerosols in composting plants using ...
Methods: A total of 124 inhalable dust samples were collected at different workplaces in 31 composting plants. Besides the determination of inhalable dust, particles, and total cell numbers, antigen quantification for moulds (Aspergillus fumigatus, Aspergillus versicolor, Penicillium chrysogenum, and Cladosporium spp.) and mites was performed.
(PDF) Plant...Extraction - ResearchGate
Grinding of plant materials. Making of fine powder with mechanical grinder. Small particles (10µm) increase the surface area. for extraction efficiency. For the …
What are some methods to detect Concentration of …
The kjeldahl method is the best analytical test to determine the concentration of proteins, but if you have not instruments to perform it, try with Bradford assay. Cite. 9th Mar, 2013. Alok Nahata ...
Determination of the silicon concentration in plant ...
The methods are linearly correlated (r 2 = 0.89), with an intercept near zero and a slope near unity. To conclude, the Tiron procedure, although originally used to assess amorphous silica in soil, can also be employed to determine the Si concentration in plants.
A Review on the Extraction Methods Use in Medicinal …
A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation.
Comparison of different methods for determining lignin ...
Aims Acid detergent lignin (ADL), acetyl bromide (AcBr), and cupric oxide oxidation (CuO) were compared as methods for determining lignin concentration and quality in plant residues. Methods These three methods were used to analyze 27 plant residues from different groups of species, i.e., legumes, crucifers, herbs, grasses, and trees. Results Median lignin …
Evaluation of diffusion and dilution methods to determine ...
Using the disk diffusion and agar dilution methods we tested the ability of bacteria to produce visible growth when certain concentration of plant extract or pure phenolic acid was added. MIC values as determined by the disk diffusion method ranged mostly from 0.313 to 40.0 mg/mL for gram-positive bacteria, indicating B. cereus as. Discussion
Micro‐method to determine iron concentrations in plant ...
The method proposed here was successfully applied to measure total Fe concentration in oven‐dried, milled plant samples. Applicability of the method for tissues other than rice and suboptimal extraction methods are discussed.
Current analytical methods for plant auxin quantification ...
The goal of this review is to summarize the recent advances (since 2000) in analytical methods for the quantification of two naturally occurring auxins, IAA and indole-3-butyric acid (IBA) in plant tissues. 2. Analytical methods for auxin quantification. Auxins are indolic acids distinguishable by a variable side chain (see Fig. 1 ).